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  • 常会庆,郑彩杰,李兆君,焦常锋,朱晓辉,何万领,王启震,吴杰.污泥施用对根际和非根际石灰性土壤中细菌多样性的影响[J].环境工程学报,2019,13(9):2250-2261.DOI:10.12030/j.cjee.201901133    [点击复制]
  • CHANG Huiqing,ZHENG Caijie,LI Zhaojun,JIAO Changfeng,ZHU Xiaohui,HE Wanling,WANG Qizhen,WU Jie.Effects of sludge addition on bacterial diversity in rhizosphere and non-rhizosphere calcareous soil[J].,2019,13(9):2250-2261.DOI:10.12030/j.cjee.201901133   [点击复制]
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污泥施用对根际和非根际石灰性土壤中细菌多样性的影响
常会庆1, 郑彩杰1, 李兆君2, 焦常锋1, 朱晓辉1, 何万领3, 王启震1, 吴杰1
0
(1.河南科技大学农学院,洛阳 471000;2.中国农业科学院农业资源与农业区划研究所,北京 100081;3.河南科技大学动物科技学院,洛阳 471000)
摘要:
为研究污泥施用对石灰性土壤细菌多样性的影响,在连续2年种植夏玉米大田系统中,施用不同量的腐熟污泥,采集根际和非根际土壤进行16S rDNA-V3-V4区高通量测序,分析污泥施用对石灰性土壤细菌丰富度、多样性指数、群落结构、功能基因在代谢途径的影响,同时对细菌群落和环境因素之间的相关性进行分析。结果表明:污泥用量分别在3.5~37.5 t·hm-2和3.5~7.5 t·hm-2有利于非根际和根际土壤OTU数量增加;但污泥在75 t·hm-2过量施用时,根际和非根际土壤细菌的Shannon多样性指数显著降低,Simpson指数显著增加,Ace,Chao丰富度指数显著降低。主成分和门水平物种丰度分析表明:污泥施用量不同会造成根际和非根际细菌群落结构差异,并且当污泥施用量为75 t·hm-2时,明显降低了非根际土壤放线菌门的丰度;污泥施用量在3.75~37.5 t·hm-2时,非根际土壤中拟杆菌门丰度会明显增加。与对照相比,当污泥用量为75 t·hm-2时,根际土壤拟杆菌门有明显的增加,增加丰度达1.45倍,但酸杆菌门和放线菌门丰度降低了49.74%和80.57%。冗余分析(RDA)表明,土壤TN、Cd、Cu、TP是影响土壤细菌群落最主要的因素,其中TN、Cd、Cu和细菌Shannon、Simpson多样性指数呈现显著相关性(P< 0.05)。由此可见,连续过量施入污泥会对根际和非根际石灰性土壤中细菌多样性造成不利影响,而且上述微生物多样性变化可作为污泥合理施用的判断依据。
关键词:  污泥  石灰性土壤  根际  非根际  细菌多样性
DOI:10.12030/j.cjee.201901133
投稿时间:2019-01-20
基金项目:国家自然科学基金面上资助项目(41571319);国家重点研发计划(2017YFD0801300);河南省科技公关项目(172102310181)
Effects of sludge addition on bacterial diversity in rhizosphere and non-rhizosphere calcareous soil
CHANG Huiqing1, ZHENG Caijie1, LI Zhaojun2, JIAO Changfeng1, ZHU Xiaohui1, HE Wanling3, WANG Qizhen1, WU Jie1
(1.School of Agriculture, Henan University of Science and Technology, Luoyang 471000, China;2.Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3.School of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China)
Abstract:
In order to study the effects of sludge application on bacterial diversity of calcareous soil, different amounts of compost sludge were applied in a 2 consecutive year-summer maize field system. The high-throughput sequencing of 16S rDNA-V3-V4 region was carried out for collected rhizosphere and non-rhizosphere soil. Effects of sludge application on bacterial richness, diversity index, community formation and functional genes were analyzed. The correlation between bacterial community and environmental factors was also determined. The results showed that the amounts of OTU in these two kinds of soil increased at sludge addition amounts of 3.5~37.5 t·hm-2 in non-rhizosphere soil and 3.5~7.5 t·hm-2 in rhizosphere soil, respectively. However, at sludge addition amounts above 75 t·hm-2, Shannon index of bacterial diversity in rhizosphere and non-rhizosphere soil decreased significantly, Simpson index increased significantly, Ace and Chao indices for bacterial abundance also decreased significantly. Principal component and phylum-level species abundance analysis showed that different sludge addition amount could cause the differences in rhizosphere and non-rhizosphere bacterial community structure, and the abundance of actinobacteria in non-rhizosphere soil was significantly reduced at the sludge addition amount of 75 t·hm-2. The abundance of Bacteroidetes in non-rhizosphere soil increased significantly at the sludge addition amounts of 3.75~37.5 t·hm-2. Compared with the control, at the sludge addition amount of 75 t·hm-2, the abundance of Bacteroidetes in rhizosphere soil increased by 1.45 times, but the abundances of Acidobacteria and Actinobacteria decreased by 49.74% and 80.57%, respectively. Redundancy analysis (RDA) showed that soil TN, Cd, Cu and TP were the most important factors affecting soil bacterial community, among which TN, Cd, Cu were significantly correlated with Shannon and Simpson diversity indexes (P<0.05). Therefore, the continuous and excessive sludge application can adversely affect bacterial diversity in rhizosphere and non-rhizosphere calcareous soil, Moreover, the changes of microbial diversity indices mentioned above can be used as a basis for the rational sludge application.
Key words:  sludge  calcareous soil  rhizosphere  non-rhizosphere  bacterial diversity